Session: 7

PS7-07 | Angiotensin II receptor´s immunolocalization in a model of Parkinson’s disease

Georgina Pulcini

Laboratorio de Biología Molecular/Facultad de Química, Bioquímica y Farmacia/ Universidad Nacional de San Luis

Parkinson’s Disease (PD) is a progressive neurodegenerative disorder characterized by the selective death of dopaminergic neurons of Substantia nigra (SN). The presence of the Angiotensin II (Ang II) receptors has been described in SN. Overstimulation of AT1 receptors could produce oxidative stress, which affect the sensitive area of the SN. We utilized a rat rotenone model, which it was assayed previously by our group, to evaluate changes in the Ang II receptor´s localization in SN. Microparticles with the neurotoxin rotenone were administered by subcutaneous injection (dose of 50 mg/kg). Both Ang II receptors were detected with anti- AT1 and anti AT2 antibodies in brain cryostat sections. Immunohistochemical evaluation of tyrosine hydroxylase indicated that treatment with rotenone microparticles did have effect on dopaminergic neurons (a reduction about 33%). The density of AT1 positive cells was significantly lower in treated rats than in control animals (p <0.05). AT1 receptors were localized mainly in a perinuclear region. While the density of AT2 positive cells showed no significant difference between treated and control animals. AT2 receptors predominantly exhibited cytoplasmatic and perinuclear localization. In coincidence with our previous results, we confirm the presence of both Ang II receptors in SN of rotenone model of PD. These findings are important to provide information about the potential role of brain renin angiotensin system in neurodegenerative processes